Deep Focus 3.1 module

Deep Focus 3.1 module is an additional software module for QuickPHOTO programs that creates images with depth of focus much higher than is the depth of focus of optical microscopes (so called z-stacking). It can be used with stereo microscopes as well as with other types of optical microscopes for observation in transmitted or reflected light. Deep Focus 3.1 module is compatible with QuickPHOTO CAMERA 2.3, MICRO 2.3 and Industrial 2.3. Deep Focus in version 3.1 uses new improved faster algorithm which produces composed images of excellent quality. The whole process can now be automatized using a module for motorized focusing.

Comparison of standard and composed image - click for detail

Functions overview:

• universality - the module can be used with:
  • stereo microscopes
  • optical microscopes for observation in transmitted light
  • optical microscopes for observation in reflected light
  • electron microscopes
  • macro images
• the possibility to automate the process of creating the images with extreme depth of focus when using the CB-ZM for motorized focusing
• easy to use and user friendly graphical interface
• possibility to compose images opened in QuickPHOTO program as well as images stored on a hard drive
• works with images with ultra-high resolution
• compatibility with QuickPHOTO CAMERA 2.3, MICRO 2.3 and Industrial 2.3 programs

Principle of function:

First the "slices" - images with different plane of focus are captured. Every image contains different parts of a specimen well focused. On the image of wasp only the upper wing is in focus.

Standard image - click for detail

Only the well focused area is used from each of the "slices" by the Deep Focus 3.1 module. The resulting completely focused image is composed from these well focused areas. Possible shift of a specimen in the field of camera view and small changes of a scale are compensated automatically.

Composed image - click for detail

Automation of the process of composing

The whole process of slices capturing and composing can be automated using the CB-ZM module for motorized focus control.
The procedure is following:
First, focus the microscope to the bottom part of the specimen and set the lower limit. Then focus the microscope to the highest part of the specimen and set the upper limit. Then just set the required number of slices or a step between slices, press the Start button and wait for the completely focused image.

Try the Deep Focus 3.1 module for free

We offer you a free of charge possibility to try the Deep Focus 3.1 module on your specimens. All you need to do is to capture the series of "slices" of your specimen and send it to us. We will send you back the image composed by the Deep Focus 3.1 module. You can send us the series by e-mail to support@promicra.com address or if it will not fit in an e-mail you can use some file sharing service e.g. www.filefactory.com and send us the download link to the same e-mail address. You can find the tips and recommendations how to capture a series here.

Supported motorized devices:

• CB-ZM - an additional module for motorized focus control of optical microscopes
• several types of motorized microscopes

The gallery of composed images:

You can display the detail by clicking on the preview.

Images from stereo microscopes:

Images from upright microscopes - transmitted light:

Images from upright microscopes - reflected light:

Minimal system requirements:

Minimal system requirements depend on the resolution of used digital imging device.

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