Deep Focus - Focus Stacking Module for QuickPHOTOCreate completely in-focus images automatically

Deep Focus - focus stacking module for QuickPHOTO - industry

Deep Focus is an extension software module for QuickPHOTO programs. It is designed for creating completely in-focus images, which due to a limited depth of field of optical microscopes cannot be acquired in a standard way. Completely in-focus images are composed from stacks of images with varying focal plane (partially focused images). Only the well-focused areas are used from each image and the resulting image is composed from them. This technique is called Extended Depth of Field imaging (EDF), z-stacking or focus stacking. The process can be automated using motorized stands or additional focus drives provided by PROMICRA and other manufacturers.

 Brochure - life science

 Brochure - industry

 User guide

Latest Deep Focus 3.5 release is 10x – 20x faster than the previous version. Updating to the version 3.5 is free of charge for users of previous versions (see Downloads section).

How It Works

Acquiring images in different focus planes

Capturing of the wasp by Deep Focus module of QuickPHOTO

Features

Deep Focus no artifacts
Excellent Quality

Excellent quality images without artifacts suitable for publication

Deep Focus fast processing
Fast Processing

Deep Focus takes full advantage of the advanced features of modern processors: use of all cores and SIMD instructions.

Deep Focus manual stacking
Manual Use

Options to compose manually acquired images opened in the QuickPHOTO program as well as images stored on a hard drive

PROMICRA motorized stands
Automation

The focus stacking process can be automated using PC-controlled motorized stands or additional focus drives.Learn more »

Different microscopes
Suitable for All Microscope Types

Deep Focus can be used with stereomicroscopes, optical microscopes for observation in transmitted or reflected light, electron microscopes as well as for macro imaging.

Deep Focus shift compensation
Shift Compensation

Specimen shift in the field of view and minor scale changes are compensated automatically during the composition process.

HDR module for QuickPHOTO - industry
EDF + HDR

Deep Focus can be combined with HDR module to produce completely in-focus HDR images.

Automation

The whole process of image stack acquisition and composition of a completely in-focus image (focus stacking) can be automated using PC-controlled motorized stands or additional focus drives. The automation makes routine work with Deep Focus module much more comfortable.

The automated procedure is very straightforward:

  1. Focus the microscope to the bottom (lowest) part of the specimen using the camera’s live view and set the lower limit of the focus range.
  2. Focus the microscope to the top (highest) part of the specimen using the camera’s live view and set the upper limit of the focus range.
  3. Select the number of images to be acquired or a step between them.
  4. Click the Start button to begin the whole process of focus stacking.
  5. Wait a while until the completely in-focus image appears.

Standard vs. Deep Focus Images Comparison

Deep Focus Sample Images

Stereomicroscope

Upright Microscope – Transmitted light

Upright Microscope – Reflected light

System Requirements

 Minimum RequirementsRecommended Specifications
ProcessorDual-core processorIntel® Core™ i5 / AMD RYZEN™ 5 or better
Operating Memory2 GB4 GB or more
Operating SystemMicrosoft® Windows® 7/8.1/10 (32bit / 64bit)Microsoft® Windows® 10 (64bit)
Display Resolution1024 x 7681920 x 1080
QuickPHOTO version3.13.2
Minimum Requirements
ProcessorIntel® Core™ i3 / AMD RYZEN™ 3
Operating Memory2 GB
Operating SystemMicrosoft® Windows® 7/8.1/10 (32bit / 64bit)
Display Resolution1024 x 768
QuickPHOTO version3.1
Recommended Specifications
ProcessorIntel® Core™ i5 / AMD RYZEN™ 5 or better
Operating Memory4 GB or more
Operating SystemMicrosoft® Windows® 10 (64bit)
Display Resolution1920 x 1080
QuickPHOTO version3.2

Related Products


Microsoft®, Windows®, Windows Vista® are registered trademarks of Microsoft Corp.
Intel®, Intel Core® are registered trademarks of Intel Corporation